The game will take place on Saturday, 29 August and be held at the Tottenham Hotspur Stadium (3pm).
The fixture will be behind closed doors due to the COVID-19 pandemic.
It will be Blues first visit to London's biggest club stadium, a breathtaking multi-purpose arena that was opened in April 2019.
The game will pit Aitor Karanka against Jose Mourinho - the man he assisted at Real Madrid for a three-year period from 2010.
Blues other pre-season fixtures ahead of the return of the Championship campaign, on the weekend of 12 September, will be announced in due course.