Blues' form over the past few months has been title-chasing.BCFC
Travelling to the West Country, Steve Spooner's side will face Bristol City who were the winners of the PDL South.
Blues' form over the past few months has been title-chasing; twelve wins in 15 games has seen them steadily rise up the table and into a second-place finish ahead of the game.
Confidence will be high following a colossal win over the Latics last time out, which meant Blues finished the season with their largest margin of victory of the campaign.
Success over the Robins would set up an encounter with the winner of the other semi-final, which is being contested by Sheffield United, who topped Blues' division and Ipswich Town, who were runners up to Bristol City in the South.
Bristol City will be amongst the favourites to be crowned the national champions after a strong season under lead Coach Alex Ball.
Their 25th match of the campaign resulted in a 4-0 victory over Wigan across the bank holiday, confirming their top-of-the-table finish in the league phase.
Impressive both offensively and at the back, the Robins were the highest scorers in the league, also boasting the tightest defence across the division as well, conceding just 35 goals.
It was post-Christmas form that really ignited their charge up the standings. From January onwards, the Robins won 10 of their 15 league fixtures, drawing twice and losing on just three occasions.
Led by Ball, the former youth academy coach at Southampton has plenty of experience bringing through youngsters at the famed Saints academy.
He made one professional appearance for the Robins during a short playing career, which came in a 2-0 away defeat away at cross-city rivals Bristol Rovers at the end of the 1999/00 season.
WE MEET AGAIN:
Bristol City Under-23s 2 Blues Under-23s 1, Tuesday 27 April
A Louis Britton lob and Sam Pearson's late strike helped the West Country outfit beat Blues at the Optima Stadium in April.
Keke Simmonds had opened the scoring for Steve Spooner's side in the 50th minute but Bristol battled back, twice dispatching in the second half.
Pearson's winner came one minute from time, a precise finish from a tight angle inside the penalty box.
Adan George was brought on as Blues pushed for a late equaliser but the points stayed in Bristol.
Referee: Alex Blake
Assistant Referee: Tom Cooke and Paul Barber
Fourth Official: Yianni Savvas